Which statement is true regarding the order of collecting a pap smear sample?

The correct understanding regarding the order of collecting a Pap smear sample is that the scraper is used first. The reason for this practice is primarily related to the effectiveness in obtaining cells for cytological examination. The scraper, typically a type of spatula or brush, is designed to collect cells from the ectocervix and the transformation zone, where cervical neoplasia typically occurs.

Using the scraper first helps ensure that the cellular sample is adequate for accurate screening. Following this, the brush is used to collect cells from the endocervical canal, which provides additional material for analysis. This sequential approach makes certain that both external and internal surfaces of the cervix are sampled appropriately, enhancing the overall diagnostic yield of the Pap test.

Using only one method would limit the sample to either the ectocervix or the endocervix, potentially missing significant cellular changes. Collecting both samples simultaneously is impractical and could lead to contamination or insufficient sampling. Consequently, the order of using the scraper first before the brush is essential for effective Pap smear collection.